36
7. Start-up
• Open the field diaphragm just enough for it to
disappear from the field of view (35d).
Note:
The condenser height adjustment depends on
the thickness of the specimen. It may be ad-
justed for different specimens.
7.3 Checking Phase Contrast Rings
If your microscope is equipped for the use of
phase contrast, the light rings that fit the objec-
tives are built into the condenser.
The light rings are already centered in the fac-
tory. However, the centering should be
rechecked.
Note:
A light ring slide which is inserted into the side
of the condenser is used for condensers without
condenser disks. Centering is not required in
this case.
Note:
When swiveling in a suitable objective for
phase contrast, the corresponding light ring
must be chosen.
The objective engraving (e.g. PH 1) indicates the
corresponding light ring (e.g. 1).
Fig. 36 Focusing Telescope
1 Adjustable eye lens
2 Clamping ring for fixing the focus position
1
2
PH LR
Fig. 37 Phase Contrast Centering Procedure
PH=phase contrast ring, LR=light ring
a Condenser in brightfield (BF) position
b Condenser in phase contrast (PH) position
Light ring (LR) not centered
c Light ring and phase ring centered
ab c
a
b
cd
Fig. 35 Köhler Illumination
a Field diaphragm not focused, not centered
b Field diaphragm focused, but not centered
c Field diaphragm focused and centered
Diameter shown here is too small
d Field diameter (light) = Field diameter (view)
(Köhler Illumination)