56
9. Contrast Methods
Fig. 60 Filter holders
Leica DM2000/3000 only:
DLF filter magazine for
attachment to micro-
scope base
Filter holder for screw
attachment on con-
denser
Leica DM2500 only:
Adapter with filter
holders between
stand and LH 107/2
Leica DM2000/3000 only:
Filter holder with 2 posi-
tions or 1 position for
attachment to micro-
scope base
Magnifications of 1.6x and 2.5x
are also possible with the CL/PH or CLP/PH, UCL
or UCLP condensers if the condenser is re-
moved completely. The field diaphragm then
takes over the function of the aperture dia-
phragm.
Note:
If the microscope is equipped for polarization,
the analyzer and polarizer as well as the lambda
plate compensator must be removed or swung
out when using other contrast methods.
9.1.1 Brightfield
• If present: click the condenser disk* into the
BF position.
• If present: pull the light ring slide* out of the
condenser.
• If present: switch the fluorescence illuminator
into an empty position or filter system A.
• Insert a transmitted light specimen.
• Rotate an appropriate objective into place.
•Movable condenser heads:
The condenser top is swung out for objec-
tive magnifications < 10x.
• Bring the image into focus using the focus dial
and set the brightness.
• For an optimal field diaphragm setting, check
the Köhler illumination (→
p. 34).
• Use suitable transmitted light filters as appli-
cable (Fig. 60).
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