61
9. Contrast Methods
Fig. 65
Fluorescence illuminator with filter block changer, shutter,
BG38, field and aperture diaphragm
9.2 Fluorescence
• Insert a suitable specimen and rotate an ap-
propriate objective into place.
• Focus the image initially in transmitted light if
appropriate.
•Switch on the incident light source at the ex-
ternal power unit.
• Open the shutter.
• Select an appropriate fluorescence filter
cube.
•Switch magnification changer, if present, to
factor 1x.
• Disengage the BG 38 filter if there is no dis-
turbing red background. Always engage the
filter for photography, however.
• Open the aperture diaphragm.
• Open the field diaphragm until the whole field
of view is just illuminated.
If necessary center the field diaphragm.
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