Olympus BX53M - Page 132

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AX9448 03 Issued in April, 2021

Distributed by

Manufactured by

Shinjuku Monolith, 2-3-1 Nishi-Shinjuku, Shinjuku-ku, Tokyo 163-0914, Japan

www.olympus-global.com

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Table of Contents3
Introduction7
Safety Precautions8
Nomenclature of Units14
List of Combinable Units15
Basic Operations of the Microscope (Brightfield Observation)20
Reflected Light Brightfield Observation Procedures20
Turning on the Main Switch22
Selecting the Illumination22
Changing between the Reflected Light Illumination and the Transmitted Light22
Illumination22
Selecting the Observation Method23
When BX3M-RLAS-S Is Combined23
When BX3M-KMA-S Is Combined23
When BX3M-RLA-S Is Combined23
When BX3M-URAS-S Is Combined24
Changing between the Eyepiece Light Path and the Camera Light Path25
Placing a Sample on the Stage25
Placing a Sample25
Adjusting the Stage Height26
Using the Y-Axis Lock27
Adjusting the Tension of the X-Axis Knob and the Y-Axis Knob27
Selecting the Objective29
Focusing30
Moving the Stage Vertically30
Replacing the Fine Focusing Knob30
Adjusting the Tension of the Coarse Focusing Knob30
Setting the Movement Limit with the Coarse Focusing Knob31
Method to Find the Approximate Focal Point Easily31
Adjusting the Brightness32
When the LED Lamp Housing Is Combined32
When the Halogen Lamp Housing Is Combined32
When the Mercury Lamp Housing Is Combined32
When the Light Source Is Combined32
Using the LIM/SET Switch33
Adjusting the Observation Tube37
Tilting Adjustment37
Using the Eye Shades37
Adjusting the Interpupillary Distance38
Adjusting the Diopter38
Adjusting the Field Diaphragm of the Reflected Light Illumination40
Using the Field Diaphragm (FS)40
Adjusting During Observation41
Centering of the Field Diaphragm (FS)42
Adjusting the Aperture Diaphragm of the Reflected Light Illumination44
Using the Aperture Diaphragm (AS)44
Adjusting During Observation45
Centering of the Aperture Diaphragm (AS)45
Centering the Mercury Burner46
Inserting the Reflected Light Illumination Filter48
Using the ND Filter Lever48
Using the Filter49
Transmitted Light Brightfield Observation Procedures52
Adjusting the Field Diaphragm of the Transmitted Light Illumination54
Using the Field Diaphragm (FS)54
Centering of the Field Diaphragm (FS)54
Inserting the Transmitted Light Illumination Filter56
Various Observation Methods58
Reflected Light Darkfield Observation Procedures58
Reflected Light Simple Polarization Observation Procedures59
Reflected Light Differential Interference Contrast (DIC) Observation60
Procedures60
Reflected Light Fluorescence Observation Procedures61
Reflected Light Infrared Observation Procedures62
Reflected Light Simultaneous Observation for BF/DF63
Transmitted Light Simple Polarization Observation Procedures64
Transmitted Light Polarization Observation Procedures65
Setting the Analyzer and the Polarizer of the Reflected Light Illumination66
When BX3M-URAS-S, BX3M-RLA-S or BX3M-KMA-S Is Combined66
When BX3M-RLAS-S Is Combined67
Inserting the DIC Slider68
Adjusting the Prism69
Opening/Closing the Shutter70
Setting the Analyzer and the Polarizer of the Transmitted Light71
Illumination71
Setting the Analyzer and the Polarizer71
Inserting the MIX Slider for Reflected Light Observation72
Turning on the Illumination of the MIX Slider for Reflected Light Observation72
Adjusting the Brightness73
Selecting the Illumination Pattern74
Using the Oil Immersion Objective75
Troubleshooting76
Optical Systems76
Electrical Systems80
Coarse/Fine Focusing90
Observation Tube90
Stage91
Specifications92
Optical Performance List <<UIS2 Series95
Assembly99
Assembly Diagram99
Assembly Procedures100
Connection with PC127
Preventive Inspection Sheet for Illumination Devices129
Proper Selection of the Power Supply Cord130

Olympus BX53M - Page 132

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