Olympus BX53M - Reflected Light Differential Interference Contrast (DIC) Observation; Procedures

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4-3
Reflected light differential interference contrast (DIC) observation procedures
Observation method selector knob of the
reflected light illuminator (page 17)
Analyzer insertion slot / polarizer insertion slot
of the reflected light illuminator (page 60)
Prism movement knob of the DIC slider (page
63)
Slider insertion slot of the nosepiece (page 62)
Select the light path. (Trinocular tube only)
6
Turn ON the main switch.
1
Light path selection lever of the trinocular
tube (page 19)
Main switch (page 16)
Stage plate (page 19)
X-axis / Y-axis knob of the stage (page 19)
Nosepiece (page 23)
Coarse / Fine focusing knob of the
microscope frame (page 24)
Brightness control knob of the microscope
frame (page 26)
Brightness control knob of the microscope
frame (page 26)
Binocular tube (page 32)
Diopter adjustment ring (page 32)
Nosepiece (page 23)
Coarse / Fine focusing knob of the
microscope frame (page 24)
Aperture diaphragm knob of the reflected
light illuminator (page 39)
Field diaphragm knob of the reflected light
illuminator (page 36)
Select the differential interference contrast
/ polarization (DIC/POL) observation.
3
Set the analyzer and the polarizer.
4
Adjust the prism of the DIC slider.
16
Set the DIC slider.
5
Place a sample on the stage.
7
Engage the 10X objective in the light path.
8
Bring the sample into focus.
9
Adjust the interpupillary distance.
Adjust the diopter.
11
12
Adjust the brightness.
10
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
14
Adjust the aperture diaphragm and the field diaphragm.
13
Adjust the brightness.
15
Start observation.
Transmitted/reflected light switch (page 16)
Select the reflected light illumination
2