Olympus BX53M - Reflected Light Fluorescence Observation Procedures

55
BX53M
4-4 Reflected light fluorescence observation procedures
Select the light path. (Trinocular tube only)
3
Open the shutter.
7
Turn ON the main switch.
1
Light path selection lever of the trinocular
tube (page 19)
Shutter of the reflected light illuminator
(page 64)
Main switch (page 16)
Stage plate (page 19)
X-axis / Y-axis knob of the stage (page 19)
Nosepiece (page 23)
Turret of the reflected light illuminator (page 17)
Coarse / Fine focusing knob of the
microscope frame (page 24)
Brightness control knob of the microscope
frame (page 26)
Brightness control knob of the microscope
frame (page 26)
Binocular tube (page 32)
Diopter adjustment ring (page 32)
Nosepiece (page 23)
Coarse / Fine focusing knob of the
microscope frame (page 24)
Aperture diaphragm knob of the reflected
light illuminator (page 39)
Field diaphragm knob of the reflected light
illuminator (page 36)
Place a sample on the stage.
4
Engage the 10X objective in the light path.
5
Engage the fluorescence mirror unit
suitable for the sample in the light path.
6
Bring the sample into focus.
8
Adjust the interpupillary distance.
Adjust the diopter.
10
11
Adjust the brightness.
9
Engage the objective with the magnification to be used
in the light path and bring the sample into focus.
13
Adjust the brightness.
14
Start observation.
Open the aperture diaphragm, and
adjust the field diaphragm.
12
Transmitted/reflected light switch (page 16)
Select the reflected light illumination
2