Secomam Basic - 7.4.1. Pump Rate (Calibration)

BASIC
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7.4.1. Pump rate (calibration)
Prepare ahead:
• 4 test tubes with 1000 µl of distilled water each,
• 1 test tube with 500µl of distilled water to use as standard.
• One graduated pipette (vol. up to 500µl)
To adjust pump volume, you need to:
• Return to « CONFIGURATION ».
• Go to menu « PUMP ».
• Use pump? « YES ».
• Exit from « CONFIGURATION » (ESC twice).
• Rinse twice consecutively (using right cursor button) to fill the tube with water.
Then:
• Again return to « CONFIGURATION »,« PUMP »,« YES ».,« PUMP RATE »
• Introduce the first tube containing 1000µl of distilled water to the aspiration nozzle.
• Press (F3) « SUCK » and « VALidate » the message of 500 µl.
The pump should have aspirated 500 µl.
• Compare the remaining volume with the volume of standard tube. Or better yet, aspirate the
remaining distilled water with the pipette and verify the volume.
• If the remaining volume is more (or less) than 500µl, press « DEL» to remove the message of
500µl on display and enter the real volume sucked by the pump using the number keys (calculation
example: initial volume of 1000 µl – remaining volume = new volume to program).
• Introduce the second tube containing 1000 µl of distilled water.
• Press (F3) « SUCK » and « VALidate ». The remaining volume should be 500µl, same as the
volume of standard tube (or verify again with the pipette). If another adjustment is to be done,
repeat this process until the instrument aspirated the right 500µl volume.
• Always validate at the end of process by (F6) «OK». (To keep previous volume, press « ESC »).
7.4.2. Delay
Is the frequency (in seconds) to program for sample introduction following one reading.
You can work faster by shortening pump aspiration delay when you are more familiar with the
system.