Secomam Basic - 8.8. WAVELENGTH PROGRAMMING; 8.9. ANALYSIS MODE PROGRAMMING; End-point with Blank Serum; End-point with Sample Blank

BASIC
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8.8. WAVELENGTH PROGRAMMING
Wavelength to use is indicated by the reagent technical sheet. You may not find the exact same
wavelength among those proposed by BASIC. In that case, select one closest to the ideal wavelength
8.9. ANALYSIS MODE PROGRAMMING
End-point with blank:
When there is only the reagent may add a parasitical color to the wavelength of the reading, it is
necessary to deduct its absorbance from such reading; this is referred to as reagent blank.
When using this mode, reading is done at the final absorbance (optical density) point (at the end of the
reaction process). Zero is adjusted using reagent alone (reagent blank) or distilled water.
Sample Conc. = (Sample Abs.- Reagent blank Abs.) x Factor
End point with sample blank:
A number of environments, in particular biological ones, may be the object of spectral interferences at
the wavelength selected for the dosage. This is the case for colored molecules (hemoglobin, bilirubin,
...). These interferences which are independent of the parameter to be measured, will be eliminated by
measuring the sample alone or with a reagent which does not trigger a coloring effect. : This is called
a sample/serum blank.
Sample Conc. = [(Sample Abs.- Sample Blk Abs.) – (Reagent Blk1 Abs.- Reagent Blk0 Abs)] x Factor
End point without
blank serum
End point with
blank serum
Enzymes kinetics
Bichromatism
Kinetics with
standards