Waters 1525M - Priming the Pump

Preparing the Pump 48
3
3.2.1 Priming the Pump
Priming the pump is necessary to ensure proper pump operation when:
• Starting the pump for the first time
• Changing eluent
• The system has been idle for some time
Required Material
Priming syringe (part number WAT010337 in Startup Kit)
Procedure
1. Power on the pump.
2. Power on the MassLynx workstation and log on to Windows.
3. Select Start > Programs > MassLynx > MassLynx V4.0. The MassLynx Main
window appears.
4. Click Status to get the LC status.
5. Click the Inlet Method button (left side of the window) to open the Inlet Editor
window.
6. Click the Inlet icon to go to the pump method.
7. Set the flow rate:
• For the 1525µ Pump, set the rate to 1.0 mL/min.
• For the 1525EF Pump, set the rate to 3.0 mL/min.
If the pump is dry, start with a lower flow rate.
8. Click Save in the Inlet Method window to save the method.
9. Fully depress the priming syringe plunger to remove all air from the syringe, then
insert the syringe into the Luer fitting at the center of the draw-off valve handle
(Figure 3-1
).
STO
P
Attention:
Do not start the pump at this point in the procedure.