Powering Off the Pump 50
3
2. Allow the eluent and the system to reach proper temperatures before pump
operation.
3. Prime the pump as described in Section 3.2.1,
Priming the Pump.
Equilibrating
1. Power on all MassLynx system instruments and any peripheral devices, such as a
printer.
2. Power on the MassLynx workstation and log on to Windows.
3. Select Start > Programs > MassLynx > MassLynx V4.0. The MassLynx Main
window appears.
4. Click the Status button to get the LC status.
5. Click the Inlet Method button (left side of the window) to open the Inlet Editor
window.
6. Click the Inlet icon to go to the pump method.
7. Set the flow rate:
• For the 1525µ Pump, set the rate to 1.0 mL/min.
• For the 1525EF Pump, set the rate to 3.0 mL/min.
If the pump is dry, start with a lower flow rate.
Note:
These flow rates may not be appropriate for all columns.
8. Click Save in the Inlet Method window to save the method.
9. Click Load Method in the Inlet Method window to download the method to the
system. This will initiate the pump and flow will begin.
10. Allow the pump to equilibrate for several minutes (the amount of time depends on
total system volume, eluent miscibility, flow rate, etc.).
11. Click the Start/Stop Pumping icon in the Inlet Method window to turn off the pump
flow.
For more information about selecting an equilibration method, refer to the MassLynx Help.
3.3 Powering Off the Pump
1. If you used buffers, purge them from the pump and other HPLC system components
with HPLC-grade water. If the pump will be idle for more than one day, purge it with
a methanol/water solution to prevent the growth of microorganisms.
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