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Waters 1525M - 4.2 Diagnostic Tests

Waters 1525M
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Maintaining the 1525 Pump 53
4
Spare Parts
Refer to Appendix B, Spare Parts and Accessories, for spare parts information. Parts not
included in Appendix B
are not recommended for replacement by the customer.
Contacting Waters Technical Service
If you encounter any problems replacing parts in the pump, contact Waters Technical
Service at 800 252-4752, U.S. and Canadian customers only. Other customers, call your
local Waters subsidiary (or Technical Service Representative) or Waters corporate
headquarters in Milford, Massachusetts 01757 (U.S.A.).
4.2 Diagnostic Tests
The following diagnostic tests can help you track system performance and prevent or
identify potential problems before they interfere with operation:
Retention time stability test
Ramp-and-decay test
4.2.1 Retention Time Stability Test
Observing retention time stability during system performance monitoring tests is useful for
determining the performance of your HPLC system and its components, including the
pump. Erratic or changing retention times could be a result of dirty or malfunctioning check
valves, worn plungers or plunger seals, air bubbles in the lines, incorrectly set flow rate,
leaks, or other pump-related problems. Be aware that other factors, such as system and
column equilibration, column age, operating temperature, and so on, can also affect
retention time stability. See your system suitability documentation for more information.
4.2.2 Ramp-and-Decay Test
Use the ramp-and-decay test to monitor check valve performance.
Required Materials
Three compression plugs (part number WAT025566 in Startup Kit)
Tissues
5/16-inch open-end wrench (in Startup Kit)
HPLC-grade methanol

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