Replacing 1525µ Plunger Seals and Plungers 58
4
9. Carefully slide the pump head assembly into position over the plunger. Make sure
the indicator rod is properly aligned to prevent damaging the plunger.
10. While holding the pump head assembly against the pump, reinstall the two pump
head mounting screws. Alternately tighten each screw, using half turns to evenly
align the assembly.
11. Using the software, navigate to the Inlet Method window. Set the Flow parameter to
0.3 mL/min, click Save, then click Load Method to start the pump. Verify that the
indicator rod moves freely.
12. Click the Stop Flow button to stop the pump.
13. Use the 1/2-inch adjustable wrench to hold the check valve housing in place.
Reconnect the inlet and outlet tubings to the pump head.
14. Prime the pump as described in Section 3.2.1 on page 48
. If you notice leaks, verify
pump head and plunger seal installation.
If necessary, repeat these procedures (removing the pump head and replacing plunger
seals) for the other pump heads.
4.3.3 Cleaning and Replacing 1525µ Plungers
The plungers are sapphire rods that require careful handling. Although cleaning the
plungers is not difficult, it is important to follow these instructions carefully to avoid
damaging the plungers. Assemble all materials and read the procedure thoroughly before
you begin.
Required Materials
• 5/16-inch open-end wrench (in Startup Kit)
• 5/32-inch Allen wrench (in Startup Kit)
• 9/64-inch Allen wrench (in Startup Kit)
• 1/2-inch adjustable wrench (in Startup Kit)
• Plunger insertion tool and seal extraction tool (in Startup Kit) (see Appendix B
for part
numbers)
• Sonicator
• HPLC-grade methanol
• Replacement plunger
• Replacement plunger seals
STO
Attention:
Hold the pump head against the pump while replacing the screws
to avoid breaking the plunger.
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