Solvent Considerations 99
C
C.3 Solvent Miscibility
Before changing solvents, refer to Tabl e C- 5 to determine the miscibility of the solvents to
be used. When changing solvents, be aware that:
• Changes involving two miscible solvents may be made directly. Changes involving
two solvents that are not totally miscible (for example, from chloroform to water),
require an intermediate solvent (such as methanol).
• Temperature affects solvent miscibility. When running a high-temperature application,
consider the effect of the higher temperature on solvent solubility.
• Buffers dissolved in water may precipitate when mixed with organic solvents.
When switching from a strong buffer to an organic solvent, flush the buffer out of the
system with distilled water before you add the organic solvent.
Table C-4 Organic Solvents for Use with the 1525 HPLC Pump
Organic Solvents
4-Cyanophenol Chloroform Ethylene glycol Methylene chloride
Acetone Cyclohexane Formaldehyde n-Propanol
Acetonitrile Cyclohexanone Heptane Phenol
Amyl acetate Dibutyl phathalate Hexane Tetrahydrofuran
(THF)
Benzaldehyde Dimethyl formamide Isooctane Toluene
Benzene Dimethyl sulfoxide Isopropanol Waters PIC
®
Reagents
Benzyl alcohol Ethanol Lysine hydrochloride Xylene
Butanol Ethyl acetate Methanol
Carbon
tetrachloride
Ethylene dichloride Methyl ethyl ketone
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