Troubleshooting 87
5
Baseline noise,
random
Eluents not properly degassed Degas eluents.
Flow erratic, pump not primed Prime the pump (see
Section 3.2.1, Priming the
Pump).
Check for air in the pump, or
for failing seals.
Eluents contaminated Use fresh eluent.
Column contaminated Clean and/or replace column.
System improperly grounded Plug into an outlet on a
different electrical circuit.
Use the power conditioner.
Recorder voltage incorrect Set the recorder to the correct
voltage.
Radio frequency noise Eliminate interference.
Straight baseline, no
peaks
No pump flow Set the pump flow rate.
Leak in eluent path Repair the leak.
Sensitivity loss Degraded, contaminated, or
improperly prepared sample
Use fresh sample.
Column contaminated Clean or replace the column.
Loss of column efficiency Clean or replace the column.
Change in eluent composition Correct eluent pH or ionic
composition.
Incorrect flow rate Change the flow rate.
Table 5-3 Correcting Chromatographic Problems (Continued)
Symptom Possible Cause Corrective Action
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