Waters ACQUITY UPLC M-Class - Page 69

4.3.2.6 Late eluting peaks fail
Suspected cause Possible solution
Inappropriate gradient method Use the correct gradient method.
Incorrect trapping conditions Use the standard trapping conditions.
Incorrect sample preparation Prepare the sample correctly.
4.3.2.7 Missing peaks
Suspected cause Possible solution
Detector problems • Confirm that the system is plumbed into the
detector with no leaks.
• Verify that the detector is operational.
No solvent flow • Confirm that there is sufficient solvent in
the bottles.
• Ensure that the solvent flow rate is >0.0,
and that the system pressure is normal.
Leak in the solvent path Inspect all fittings and replace as necessary.
No spray See also: The Universal NanoFlow Sprayer
Installation and Maintenance Guide on the
documentation CD.
Incorrect vial position Ensure that the vial is in the correct position.
Injector not injecting • Prime the sample syringe.
• Consult the Console online Help.
4.3.2.8 Peak shape and area issues
Suspected cause Possible solution
Incorrect detector settings See also: The applicable detector overview
and maintenance guide on the documentation
CD and the online Help.
Injection volume/sample concentration too
high; disrupting column equilibration
• Reduce the injection volume.
• Dilute the sample.
November 26, 2019, 715003588 Revision C
Page 69