1.2.9 Software...............................................................................................................................19
1.2.10 Options ..............................................................................................................................19
1.3 Additional information....................................................................................................................20
2 Performance optimization........................................................................................22
2.1 Best practices to improve performance.........................................................................................22
2.1.1 Autozeroing the flow transducers ........................................................................................22
2.1.2 Flow ramping .......................................................................................................................22
2.1.3 Flow rate on the B flow controller .......................................................................................23
2.1.4 Initial gradient hold...............................................................................................................23
2.1.5 Trapping flow rate and trapping composition.......................................................................23
2.1.6 Re-equilibration of column at the end of analysis ...............................................................24
2.1.7 System equilibration after auto zero ....................................................................................24
2.1.8 Re-equilibration between methods .....................................................................................24
2.1.9 Temperature sensitivity........................................................................................................24
2.2 General guidelines for a microscale system .................................................................................25
2.2.1 Required solvents................................................................................................................25
2.3 Dispersion .....................................................................................................................................26
2.4 Carryover ......................................................................................................................................26
2.5 Contamination ...............................................................................................................................27
2.5.1 Contamination when operating with neutral or basic mobile phases................................... 28
2.6 Preventing leaks............................................................................................................................29
2.6.1 Installing tubing and fittings .................................................................................................29
2.7 Sample preparation.......................................................................................................................47
3 Preparation and operation......................................................................................48
3.1 Preparing the system ....................................................................................................................48
3.1.1 Powering-on the system hardware ......................................................................................48
3.1.2 Configuring the system software .........................................................................................49
3.1.3 Monitoring LEDs ..................................................................................................................49
3.1.4 Power LED...........................................................................................................................49
3.1.5 Status LEDs.........................................................................................................................49
3.2 Starting the instrument console.....................................................................................................51
3.3 Instrument control panels..............................................................................................................52
3.3.1 Solvent manager control panels ..........................................................................................52
November 26, 2019, 715003588 Revision C
Page ix
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