MS/MS operating modes 1-11
MS/MS operating modes
The following table shows the MS/MS operating modes.
Product (daughter) ion mode
Product ion mode is the most commonly used MS/MS operating mode. An ion
of interest is selected for fragmentation in the collision cell, thus yielding
structural information.
Product ion mode:
Typical applications
• Structural elucidation (for example, peptide sequencing)
MS/MS operating modes :
Operating mode MS1 Collision cell MS2
Product
(daughter) ion
spectrum
Static (at
precursor mass)
Pass all masses Scanning
Precursor
(parent) ion
spectrum
Scanning Static (at product
mass)
MRM Static (at
precursor mass)
Static (at product
mass)
Constant neutral
loss spectrum
Scanning
(synchronized
with MS2)
Scanning
(synchronized
with MS1)
MS1
Static (at precursor mass)
MS2
Scanning
Collision cell
Pass all masses
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