Waters TQ Detector Quick Start Guide

Developing experiment methods 4-9
Developing experiment methods
IntelliStart allows you to automatically develop quantitative MRM or SIR
methods for compounds of interest. Up to four compounds can be handled in a
single process. Up to three transitions can be selected for each compound.In
this example, methods for sulfadimethoxine are created.
Required materials
Sulfadimethoxine solution (100 pg/µL)
To create a method:
1. Prepare the IntelliStart fluidics system with sulfadimethoxine solution
in reservoir B.
See also: “To prepare the IntelliStart fluidics system:” on page 4-2.
2. In the ACQUITY UPLC Console system tree, click TQ Detector.
3. Ensure that the Ion Mode is ES+.
4. In the ACQUITY UPLC Console system tree, click IntelliStart.
5. Clear the Instrument Setup check box.
6. Click Develop Method.
7. If you require system pre-checking, click Pre-checks.
See also: TQ Detector online Help topic “IntelliStart flow diagram”.
8. Click Start .
9. In the IntelliStart Setup Parameters dialog box, click the Method
Developer tab.
Note: The masses, tune file and IntelliStart fluidics system parameters
already set in IntelliStart are used.
10. Select the parameters shown in the following figure.

Waters TQ Detector Specifications

General

Brand	Waters
Model	TQ Detector
Category	Measuring Instruments
Language	English