OPERATION
Axiovert 200 Illumination and contrasting techniques Carl Zeiss
B 40-080 e 03/01 3-39
The specimen field with the relevant pupil image
of the VAREL diaphragm is shown in Fig. 3-22. In
the pupil image, the diaphragm appears rotated by
180°.
☞
− Shifting the VAREL illumination to outside the pupil corresponds to unilateral darkfield
illumination.
− Shifting the VAREL illumination between the Ph and VAREL rings of the objective
corresponds to oblique brightfield illumination.
Fig. 3-23 Pupil images in VAREL contrast
Fig. 3-22 VAREL contrast with microtiter
plates
Ph ring VAREL ring VAREL diaphragm
Objective Var contrast
Oblique brightfield
illumination
Unilateral darkfield
illumination
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