Acea RTCA SP Instrument - E-Plate 96 and RTCA Resistor Plate 96

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System Description
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2.4 E-Plate 96 and RTCA Resistor Plate 96
E-Plates 96 are single use, disposable devices used for performing cell-based assays on
the RTCA SP Instrument.  e E-Plate 96 is similar to commonly used 96x well micro-
titer plates. Each well of the 96 wells on the E-Plates 96 has incorporated sensor electrode
arrays so that cells inside each well can be monitored and assayed.
 e plate has a low evaporation lid design.  e bottom diameter of each well is 5.0 mm
±0.05 mm, with a total volume of 243 μl ± 5 μl. Approx. 80% of the bottom area of each
well are covered by the electrodes.  e plate is designed to be used in an environment of
+15°C to +40°C, relative humidity 98% maximum without condensation.
Figure 10: The E-Plate 96.
For system veri cation purpose, the RTCA Resistor Plate 96 is used with the RTCA SP
Instrument as a standard accessory (see section on Resistor Plate Veri cation of the RTCA SP
Instrument).  e RTCA Resistor Plate 96 has the same size as the E-Plate 96. Instead of a micro
sensor array, an array of resistors is placed at each well position in such a way that it shows
resistance values close to that of an E-Plate 96 when only cell media are put into each well.
System functionality can be veri ed by putting the RTCA Resistor Plate 96 in the
RTCA SP Station.
RTCA Resistor Plates 96 are not instrument-speci c. Each RTCA Resistor Plates 96 can be
used to perform Resistor Plate Veri cation on each RTCA SP Station or RTCA MP Station.
Figure 11: The RTCA Resistor Plate 96.
System Description
E-Plate 96 and RTCA Resistor Plate 96