Acea RTCA SP Instrument - I DID a Background Measurement. the RTCA Software Indicated that Many Wells May Have Background Problems. What Should I Do

57
Appendix
E
E
1.8 I did a background measurement. The RTCA Software
indicated that many wells may have background
problems. What should I do?
Please remove the E-Plate 96 from the RTCA SP Station, ensure that the contact pads on
the E-Plate 96 are clean and re-insert the E-Plate 96.  en perform Scan Plate. Check
the Message page for results to see whether you have many wells showing connection
problems. You can also check the plate scanning data shown on the Cell Index Page. You
can check whether any measured well values show an open circuit (i.e., the resistance
values are abnormally high, for example, more than 500 ohm) or are negative. If the scan-
plate Message page shows Plate Scanned. Connections ok, then you should be able to re-
start the experiment. Note that the RTCA So ware may still indicate that a few wells may
have background problems since the so ware has strict criteria for determining whether a
well has a good background. A well that is indicated as “may have background problems”
does not always cause an experimental problem.
If the message indicates connection problems with many wells or if the plate-scanning
data  le shows many wells having open circuits, there may be problems with the E-Plate
96 or RTCA SP Station or both. Please perform an additional Scan Plate a er removing
the E-Plate 96 in question from the RTCA SP Station and then re-inserting/repositioning
it in the Station. Check the Scan Plate results again, as described above, to see whether the
results for the wells concerned are similar to previous results. If problems do not occur in
the 2nd Scan Plate, experiments can be continued.  e initial issue may be related to the
insertion or positioning of the E-Plate 96 into the RTCA SP Station.
If the results for wells with connection problems or open-circuit wells are similar to previ-
ous results, the E-Plate 96, the RTCA SP Station or both have problems.
First, please check to see whether the E-Plate 96 or RTCA SP Station is damaged. If so,
contact your local ACEA representative.
Second, if there is no visible damage, see whether all connector pads on the E-Plate 96 are
clean and free of dust/dirt particles. If not, clean the connector pads on the E-Plate 96 with
a tissue wetted with 80% alcohol. A er this cleaning, re-insert and reposition the E-Plate
96 for another Scan Plate test. If the number of open-circuit wells is the same as before,
then please report the problems to your local ACEA representative.
Troubleshooting
I did a background measurement. The RTCA Software indicated that many wells may have background problems.
What should I do?
57
Appendix
E
E
E
E
E
E
E
E
E
E
1.8 I did a background measurement. The RTCA Software
indicated that many wells may have background
problems. What should I do?
Please remove the E-Plate 96 from the RTCA SP Station, ensure that the contact pads on
the E-Plate 96 are clean and re-insert the E-Plate 96.  en perform
Scan Plate
. Check
the
Message
page for results to see whether you have many wells showing connection
problems. You can also check the plate scanning data shown on the
Cell Index
Page. You
Cell Index Page. You Cell Index
can check whether any measured well values show an open circuit (
i.e.
, the resistance
values are abnormally high, for example, more than 500 ohm) or are negative. If the scan-
plate
Message
page shows
Plate Scanned
.
Connections
ok
, then you should be able to re-
start the experiment. Note that the RTCA So ware may still indicate that a few wells may
have background problems since the so ware has strict criteria for determining whether a
well has a good background. A well that is indicated as “may have background problems”
does not always cause an experimental problem.
If the message indicates connection problems with many wells or if the plate-scanning
data  le shows many wells having open circuits, there may be problems with the E-Plate
96 or RTCA SP Station or both. Please perform an additional
Scan Plate
a er removing
the E-Plate 96 in question from the RTCA SP Station and then re-inserting/repositioning
it in the Station. Check the
Scan Plate
results again, as described above, to see whether the
results for the wells concerned are similar to previous results. If problems do not occur in
the 2nd
Scan Plate
, experiments can be continued.  e initial issue may be related to the
insertion or positioning of the E-Plate 96 into the RTCA SP Station.
If the results for wells with connection problems or open-circuit wells are similar to previ-
ous results, the E-Plate 96, the RTCA SP Station or both have problems.
First, please check to see whether the E-Plate 96 or RTCA SP Station is damaged. If so,
contact your local ACEA representative.
Second, if there is no visible damage, see whether all connector pads on the E-Plate 96 are
clean and free of dust/dirt particles. If not, clean the connector pads on the E-Plate 96 with
a tissue wetted with 80% alcohol. A er this cleaning, re-insert and reposition the E-Plate
96 for another
Scan Plate
test. If the number of open-circuit wells is the same as before,
then please report the problems to your local ACEA representative.
Troubleshooting
I did a background measurement. The RTCA Software indicated that many wells may have background problems.
What should I do?