55
Appendix
E
E
1.4 How do I reinitialize the RTCA SP Instrument after a
power failure?
When the power is restored, restart the RTCA Control Unit. Do not open the RTCA
Software at this time.
Turn off the RTCA Analyzer. Wait for 2-3 seconds, and then turn it back on.
Open the RTCA Software and locate the data fi le that was generated before and
during the power failure. Open the fi le.
Click the Continue button in the top left corner of the RTCA Software. The experiment
starts where it left off when power was interrupted.
1.5 The Cell Index signal is signifi cantly lower than usual.
Why?
You should take the E-Plate 96 out of the incubator and check the cell status (cell number,
cell distribution and morphology) under a microscope. e following are possible reasons:
Maybe the initial seeding cell number was not correct.
Maybe you forgot the step of “leave the E-Plate 96 at room temperature for about 30
minutes” a er the cells were seeded. If that step was not done, then cell distribution
might not be uniform over the well surface. e cell index signals would be low,
especially for the corner wells.
Maybe there is some type of contamination and cells are dying.
Maybe the cells were harvested from an over-con uent ask. Our experience is that if
the cells in the ask reached a con uence over 80%, then the cells may not be in the
Log phase and the cell status may not be ideal. Such cells may give a low cell index
signal when seeded into an E-Plate 96.
Maybe there is something wrong with the incubator. One likely symptom of incubator
problems is that the cell index is lower on edge wells than on the center wells, or the
cell index drops to nearly zero faster on the edges than the center wells. e problems
with incubators may include CO
2
level, temperature control, humidity control and
certain chemicals released from the incubator. We have encountered several instances
where the incubator itself was presenting to be “toxic” or a ecting cell growth. Some-
times a simple but thorough cleaning of the incubator interior may be su cient to
solve the problems. Alternatively, simply running the experiment in another incubator
may solve the problems.
Please make sure, when removing the E-Plate 96 from the incubator, that the instrument
is not performing a measurement. If the instrument is waiting for next sweep, make sure
that you have enough time to check the cells under a microscope. If you do not have time,
you may have to stop the current step and then take the device out of the incubator for
assessment. en Resume the experiment a er putting the E-Plate 96 back in.
Troubleshooting
How do I reinitialize the RTCA SP Instrument after a power failure?
1.4 How do I reinitialize the RTCA SP Instrument after a
When the power is restored, restart the RTCA Control Unit. Do not open the RTCA
Turn off the RTCA Analyzer. Wait for 2-3 seconds, and then turn it back on.
Open the RTCA Software and locate the data fi le that was generated before and
during the power failure. Open the fi le.
button in the top left corner of the RTCA Software. The experiment
Continue button in the top left corner of the RTCA Software. The experiment Continue
starts where it left off when power was interrupted.
1.5 The Cell Index signal is signifi cantly lower than usual.
You should take the E-Plate 96 out of the incubator and check the cell status (cell number,
cell distribution and morphology) under a microscope. e following are possible reasons:
Maybe the initial seeding cell number was not correct.
Maybe you forgot the step of “leave the E-Plate 96 at room temperature for about 30
minutes” a er the cells were seeded. If that step was not done, then cell distribution
might not be uniform over the well surface. e cell index signals would be low,
especially for the corner wells.
Maybe there is some type of contamination and cells are dying.
Maybe the cells were harvested from an over-con uent ask. Our experience is that if
the cells in the ask reached a con uence over 80%, then the cells may not be in the
Log phase and the cell status may not be ideal. Such cells may give a low cell index
signal when seeded into an E-Plate 96.
Maybe there is something wrong with the incubator. One likely symptom of incubator
problems is that the cell index is lower on edge wells than on the center wells, or the
cell index drops to nearly zero faster on the edges than the center wells. e problems
with incubators may include CO
level, temperature control, humidity control and
certain chemicals released from the incubator. We have encountered several instances
where the incubator itself was presenting to be “toxic” or a ecting cell growth. Some-
times a simple but thorough cleaning of the incubator interior may be su cient to
solve the problems. Alternatively, simply running the experiment in another incubator
Please make sure, when removing the E-Plate 96 from the incubator, that the instrument
is not performing a measurement. If the instrument is
that you have enough time to check the cells under a microscope. If you do not have time,
you may have to stop the current step and then take the device out of the incubator for
the experiment a er putting the E-Plate 96 back in.
How do I reinitialize the RTCA SP Instrument after a power failure?
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