Agilent 1100 Series LC/MSD Trap System Quick Start Guide 21
Step 2—Prepare the instrument
Read and follow the steps in the user information listed below to learn how to 
prepare the instrument for a run.
• Chapter 1, “Prepare for the analysis”, in the Familiarization Guide to learn to 
prepare the LC and Trap to run an ESI demo sample. This chapter includes 
exercises to help you learn to calibrate and tune the instrument.
• Online Help for the tasks listed in the roadmap below and descriptions of the 
fields found in the windows and dialog boxes needed for these tasks
Start 
here!
Prepare the 
instrument
3 Do an 
isolation 
calibration
4 Do a 
fragmentation 
calibration
5 Optimize 
sensitivity 
(MS)
1 Prepare the 
LC to run the 
sample
2 Do a scan 
calibration
6 Optimize 
fragmentation 
(MS/MS)