1260 Infinity II Analytical-Scale & Bio-inert Fraction Collector User Manual 49
Using the Module
3
Configuration and Operation of the Fraction Collector
Special Applications
Pooling
We define pooling as collecting fractions from multiple injections into the
same fraction locations. In the Agilent ChemStation, there are two ways to
initiate pooling:
• If you want to collect multiple injections from the one sample vial or sample
well into the same fraction location, specify multiple injections in the
Sequence Table.
• In case you want to collect multiple injections from the several sample vials
or sample wells into the same fraction location, specify the same Fract. Start
for multiple samples in the Sequence Table. An example is displayed in
Figure 9 on page 49. In this sequence the sample 1 will be injected from
plate 1 position A1 of the Autosampler and fraction collection will start at
plate 1 position A1 of the fraction collector.
For the next sample 2, which is injected from a different location in the high
performance autosampler, the fraction collection will start again at plate 1
position A1 of the fraction collector.
Figure 9 Pooling sequence
Data loss due to pump shut-down
If you specify multiple injections for pooling, the overfill protection is not activated. If a
fraction collection location is overfilled, an error message occurs and the pump is shut
OFF.
➔ Make sure that all fraction collection locations are large enough to completely
collect all pooled fractions.
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