Installation and Verification of Other Sources 4
To verify the operation of the G1947B APCI source
Agilent 6400 Series Triple Quad LC/MS System Installation Guide 75
loosen the lock nut, adjust the latch to the proper fit, and then tighten
the lock nut so that the latch maintains its adjustment.
f Connect the APCI corona and vaporizer heater cables to the connector
on the left side panel of the instrument.
g Connect the 1/8-inch nebulizing gas tubing from the LC/MS mainframe
to the nebulizer gas fitting.
h Connect the PEEK tubing from the selection valve (inside the front
cover) to the top of the nebulizer.
To verify the operation of the G1947B APCI source
There is no sensitivity checkout with the G1947B APCI source on the Triple
Quadrupole LC/MS. To verify its proper operation, run a manual tune with the
G2432A APCI/APPI Calibration Solution.
Note that Auto Tune is not supported with this source.
1 Install the G2432A APCI/APPI Calibration Solution.
2 Check the tuning of the APCI source:
a In Data Acquisition program, set Context to Tune.
b Open the auto tune file that was generated with the electrospray or
multimode source.
c In the Manual Tune tab, mark the Calibrant on check box, and then
acquire the data.
d Verify that the masses in the MS1 and MS2 tabs are correct for the
loaded source before you manually tune the peaks.
e Verify that you have sufficient abundance for the tune peaks, that the
tune peaks have peak widths of 0.7 ±0.05 amu, and that mass
assignments are within ±0.15 amu of the target mass assignments.
If needed, click Adjust Gain & Offset to establish the Mass Axis and
Peak Width values. Repeat these for both MS1 and MS2 tabs.
3 Save the tune file.
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