6 Reference
Positive Ion Mode Checkout Conditions
106 Agilent 6400 Series Triple Quad LC/MS System Installation Guide
Positive Ion Mode Checkout Conditions
These conditions are used in a Positive Ion Mode checkout.
Tabl e 1 1 HPLC Pump
Time %A (water w/0.1%
formic acid)
%B (acetonitrile) Flow
Initial90100.4 mL/min
0.190100.4 mL/min
12980.4 mL/min
22980.4 mL/min
2.01 90 10 0.4 mL/min
4Stop
Tabl e 1 2 HPLC Autosampler
Injection volume 2 µL
Autosampler temperature 4°C
Needle wash flushport 75:25 methanol:water
Needle wash location Flushport, 10 seconds
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