4 Installation and Verification of Other Sources
To verify the operation of the G1978B multimode source
78 Agilent 6400 Series Triple Quad LC/MS System Installation Guide
To verify the operation of the G1978B multimode source
Note that Auto Tune is not supported with this source.
1 Install the G1969-85020 MMI-L Low Concentration Tuning Mix.
2 Check the tuning of the multimode source:
a In Data Acquisition program, set Context to Tune.
b Open the auto tune file that was generated with the electrospray or
multimode source.
c In the Manual Tune tab, mark the Calibrant on check box, and then
acquire the data.
d Verify that the masses in the MS1 and MS2 tabs are correct for the
loaded source before you manually tune the peaks.
e Verify that you have sufficient abundance for the tune peaks, that the
tune peaks have peak widths of 0.7 ±0.05 amu, and that mass
assignments are within ±0.15 amu of the target mass assignments.
If needed, click Adjust Gain & Offset to establish the Mass Axis and
Peak Width values. Repeat these for both MS1 and MS2 tabs.
3 Save the tune file.
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