72 1200 Series DAD and MWD User Manual
5 How to optimize the Detector
Traditionally LC analysis with UV detectors is based on comparing
measurements with internal or external standards. To check photometric
accuracy of the Agilent 1200 DAD/MWD it is necessary to have more precise
information on path lengths of the flow cells.
The correct response is:
expected response * correction factor
Please find below the details of the Agilent 1200 DAD/MWD flow cells:
Figure 26 Influence of Cell Path Length on Signal Height
Absorbance
Time (min)
Analysis of pesticide standard
10-mm optical path length
6-mm optical path length
Tab le 12 Correction factors for Agilent 1200 DAD/MWD flow cells
Flow cell type Cell volume Part number Path length
(nominal)
Path length
(actual)
Correction
factor
Standard flow cell 13 µl G1315-60022 10 mm 9.80 ± 0.07 mm 10/9.8
Semi-micro flow cell 5 µl G1315-60025 6 mm 5.80 ± 0.07 mm 6/5.8
Micro flow cell 2 µl G1315-60024 3 mm 3.00 + 0.05 mm /
-0.07 mm
3/3
500 Nanoliter flow cell kit 0.5 µl G1315-68724 10 mm 10.00 ± 0.02 mm 10/10
80 Nanoliter flow cell kit 0.08 µl G1315-68716 6 mm 6.00 ± 0.02 mm 6/6
High Pressure flow cell 1.7 µl G1315-60015 6 mm 5.75 ± 0.07 mm 6/5.75
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