Agilent InfinityLab LC Series Vialsamplers User Manual 168
5 Optimizing Performance
Optimization for Lowest Carryover
Using the Needle Wash in the Wash Vial
For very critical applications where the outside of the needle cannot be cleaned
sufficiently with one wash port solvent you can use an injector program and an
additional wash vial with an appropriate and stronger solvent for cleaning. The
wash vial should contain solvent in which the sample components are soluble,
and the vial should not be capped. If the wash vial is capped, small amounts of
sample remain on the surface of the septum, which may be carried on the needle
to the next sample.
Injector Program with Needle Wash
The injector program includes the command WASH. When this command is
included in the injector program, the needle is lowered once into the specified
wash vial or flushport before injection.
For example:
1 WASH > Location P2-A1 or flushport
2 Draw 5 µL from Sample Location P1-A1
3 INJECT
Line 1 moves the needle to vial P2-A1 or the flushport. Line 2 draws 5 µL from the
current sample vial P1-A1. Line 3 injects the sample (valve switches to main
pass).
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