Agilent InfinityLab LC Series Vialsamplers User Manual 43
2 Site Requirements and Specifications
Performance Specifications
Carryover <0.004 % (40 ppm) with needle
wash
Using the following conditions:
• Column: Agilent Pursuit XRs
3 C18, 2.0 x 50
mm
• Mobile Phase:
• A: 0.1 % TFA in water
• B: 0.1 % TFA in acetoni-
trile
• Isocratic : %B = 40 %
• Flow rate: 0.5 mL/min
• Temperature: Ambient
• Wavelength: 257/4 nm
• Injection volume: 1 µL
• Sample: 1200 ng/µL Chlor-
hexidine for UV (dissolved
with mobile phase A), 1
µL
injected and measured with
Agilent UV detector
• Wash solution: H
2
O with
0.1
% TFA (3 s)
Injection cycle time 18 s for draw speed 200 µL/min
Ejection speed: 200 µL/min
Injection volume: 1 µL
Minimum sample vol-
ume
1 µL from 5 µL sample in 100 µL
microvial, or 1
µL from 10 µL
sample in 300
µL microvial.
Needle height offset has to be
adapted to ensure that needle
doesn’t touch vial bottom.
Default needle height = 0
equates to 2
mm above the vial
bottom.
Instrument control LC & CE Drivers A.02.12 or
above
Instrument Control Framework
(ICF) A.02.03 or above
Instant Pilot (G4208A) with firm-
ware B.02.19 or above
Lab Advisor B.02.07 or above
For details about supported
software versions refer to the
compatibility matrix of your ver
-
sion of the LC & CE Drivers
Communication Controller Area Network (CAN),
Local Area Network (LAN),
ERI: ready, start, stop and
shut-down signals
Table 3 Performance Specifications G7129B
Type Specification Comments Method/Conditions
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