Contents
NovoCyte Quanteon Flow Cytometer Operator's Guide 7
Connect NovoCyte Fluidics Cart (Optional) 55
Connect NovoSampler Q (Optional) 55
Connect Instrument to Power Supply 56
4 Instrument Operation 57
Instrument Start-up 57
Pre-Inspection 57
Power on the Instrument 57
Turn on Workstation 58
Add Instrument Reagents 59
Empty Waste 60
Verify and Modify Instrument Configuration 61
View and Verify Instrument Configuration 61
Modify Instrument Configuration 61
Instrument QC Test 68
Instrument Shutdown 68
5 Running Samples 71
Overview 71
Create Experiment 72
Define Sample Acquisition Parameters 74
Set Sample Acquisition Conditions 76
Stop Condition 76
Flow Rate 77
Threshold 77
Run Sample 80
Perform Fluorescence Compensation 82
Automatic Fluorescence Compensation 82