Agilent Technologies NovoCyte Quanteon - Page 70

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Instrument Operation

70 NovoCyte Quanteon Flow Cytometer Operator's Guide

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In this Guide3
Table of Contents5
Contents5
Prologue11
Using this Guide11
Symbols11
Glossary14
Safety and Limitations16
General Safety17
Ergonomics19
Electrical Safety20
Laser Safety20
Laser Product Classification21
Biosafety21
Limitations22
Agilent Regulatory Compliance Statement23
CE Compliance23
Electromagnetic Compatibility23
EMC Declaration for South Korea25
Detachable Power Cord Declaration for Japan25
Sound Emission Certification for Federal Republic of Germany25
Waste Electrical and Electronic Equipment (WEEE) Directive25
Regulatory Compliance Identification Number26
Technical Support26
Introduction29
Overview29
Basic Principle29
Optics System29
Fluidics System37
Electronics System41
System Requirements43
Software43
Workstation43
Fluidics Station43
Compatible Sample Tubes44
Instrument Reagents44
QC Particles44
System Components44
Novocyte Quanteon Instrument45
Novocyte Workstation and Monitor46
Novocyte Fluidics Station II47
Novocyte Fluidics Cart (Optional)49
Novosampler Q (Optional)49
Installation51
Installation Warnings51
Space Requirements52
Environmental Requirements52
Instruction for Lifting and Carrying52
Install Novocyte Quanteon53
Connect Novocyte Workstation54
Connect Novocyte Fluidics Station II54
Connect Novocyte Fluidics Cart (Optional)55
Connect Novosampler Q (Optional)55
Connect Instrument to Power Supply56
Instrument Operation57
Instrument Start-Up57
Pre-Inspection57
Power on the Instrument57
Turn on Workstation58
Add Instrument Reagents59
Empty Waste60
Verify and Modify Instrument Configuration61
View and Verify Instrument Configuration61
Modify Instrument Configuration61
Instrument QC Test68
Instrument Shutdown68
Running Samples71
Overview71
Create Experiment72
Set Sample Acquisition Conditions76
Stop Condition76
Flow Rate77
Threshold77
Run Sample80
Perform Fluorescence Compensation82
Automatic Fluorescence Compensation82
Manual Fluorescence Compensation92
Analyze Data97
Append Data101
Delete Data102
Save Data102
Import and Export Files103
System Maintenance107
Scheduled Maintenance107
Preventative Maintenance108
Replacing Fluidic System Consumables113
Replace 0.05 Μ M Sheath Fluid Ultrafiltration (UF) Filter (Optional)122
Unscheduled Maintenance125
Debubble125
Clean the External Surface of the Instrument126
Decontamination126
Prime Fluidic System before Long-Term Storage127
Prime Fluidic System after Long-Term Storage128
Purge Fluidic System before Shipment128
Calibrate Novocyte Fluidics Station II130
Clean the Optical Filters131
Clean Fluidic System132
Troubleshooting133
Version History137
Appendix A: Novocyte Quanteon Laser and Detection Channel Configuration139
Laser Configuration139
Appendix B: Novocyte Quanteon Technical Specifications143
Novocyte Quanteon Technical Specifications143
Novocyte Workstation and Monitor Specifications146
Novocyte Fluidics Station II Specifications146

Agilent Technologies NovoCyte Quanteon - Page 70

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