Chapter 5 Analyze the Experiment
View the Multicomponent Plot
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
6. Observe the FAM dye signal. In the example experiment, the FAM dye signal
increases throughout the PCR process, which indicates normal amplification.
7. Select the negative control wells one at a time and check for amplification. In the
example experiment, there is no amplification in the negative control wells.
2b
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2a
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