Applied Biosystems 7500 Fast Getting Started

Glossary
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
112
data collection A process during the instrument run in which an instrument component detects
fluorescence data from each well of the reaction plate. The instrument transforms the signal
to electronic data, and the data are saved in the experiment file. In the 7500/7500 Fast
system software, a data collection point is indicated by an icon in the thermal profile:
• Data collection on:
• Data collection off:
delta Rn ( ∆ Rn) See baseline-corrected normalized reporter (DRn).
derivative reporter
(
− Rn ′ )
The negative first-derivative of the normalized fluorescence generated by the reporter
during PCR amplification. In the derivative reporter (–Rn
′ ) vs. temperature melt curve, the
derivative reporter signal is displayed in the y-axis.
Design Wizard A feature in the 7500/7500 Fast system software that helps you set up your experiment by
guiding you through best practices as you enter your experiment design.
diluent A reagent used to dilute a sample or standard before adding it to the PCR reaction. The
diluent can be water or buffer.
Diluted Sample
Concentration (10
✕
for Reaction Mix)
In the 7500/7500 Fast system software, a field displayed on the Sample Dilution
Calculations tab of the Reaction Setup screen. For this field, enter the sample concentration
you want to use to add to the reaction mix for all samples in the experiment. “10
✕ for
Reaction Mix” indicates that the software assumes the sample or standard component of the
reaction mix is at a 10
✕ concentration. For example, if the diluted sample concentration is
50.0 ng/
µ L (10 ✕ ), the final sample concentration in the reaction is 5 ng/ µ L (1 ✕ ).
dilution factor See serial factor.
dissociation curve See melt curve.
EFF% See amplification efficiency (EFF%).
endogenous control A target or gene that should be expressed at similar levels in all samples you are testing.
Endogenous controls are used in relative standard curve and comparative C
T
( ∆∆ C
T
)
experiments to normalize fluorescence for the target you are quantifying. Housekeeping
genes can be used as endogenous controls. See also housekeeping gene.
endpoint read See post-PCR read.
experiment Refers to the entire process of performing a run using the 7500/7500 Fast system, including
setup, run, and analysis. The types of experiments you can perform using the
7500/7500 Fast systems:
• Quantitation - standard curve
• Quantitation - relative standard curve
• Quantitation - comparative C
T
( ∆∆ C
T
)
• Melt curve
•Genotyping
• Presence/absence

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English