Applied Biosystems 7500 Fast Getting Started

Glossary
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
120
system dye Dye supplied by Applied Biosystems and precalibrated on the 7500/7500 Fast system.
Before you use system dyes in your experiments, make sure the system dye calibration is
current in the Instrument Maintenance Manager.
System dyes of the 7500/7500 Fast system include:
•FAM
™
dye
•JOE
™
dye
•NED
™
dye
•ROX
™
dye
•SYBR
®
Green dye
•TAMRA
™
dye
•VIC
®
dye
•CY3 dye
•CY5 dye
•TEXAS RED
®
dye
TaqMan
®
reagents PCR reaction components that consist of primers designed to amplify the target and a
TaqMan
®
probe designed to detect amplification of the target.
target The nucleic acid sequence that you want to amplify and detect.
target color In the 7500/7500 Fast system software, a color assigned to a target to identify the target in
the plate layout and analysis plots.
Target Library In the 7500/7500 Fast system software, a collection of targets to add to experiments. The
targets in the library contain the target name, reporter, quencher, and target color. The target
in the library may also contain comments about the target.
task In the 7500/7500 Fast system software, the type of reaction performed in the well for the
target or SNP assay. Available tasks:
• Unknown
• Negative Control
• Standard (standard curve and relative standard curve experiments)
• Positive control (genotyping experiments)
• IPC (presence/absence experiments)
• Blocked IPC (presence/absence experiments)
technical replicates Identical reactions that contain identical components and volumes and evaluate the same
sample.
See also biological replicates.

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English