Applied Biosystems 7500 Fast Getting Started

Glossary
119
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
standard curve In standard curve and relative standard curve experiments:
• The best-fit line in a plot of the C
T
values from the standard reactions plotted against
standard quantities. See also regression line.
• A set of standards containing a range of known quantities. Results from the standard
curve reactions are used to generate the standard curve. The standard curve is defined
by the number of points in the dilution series, the number of standard replicates, the
starting quantity, and the serial factor. See also standard dilution series.
standard curve
method
Method for determining absolute target quantity in samples. With the standard curve
method, the 7500/7500 Fast system software measures amplification of the target in
samples and in a standard dilution series. Data from the standard dilution series are used to
generate the standard curve. Using the standard curve, the software interpolates the absolute
quantity of target in the samples. See also standard and standard curve.
standard dilution
series
In standard curve and relative standard curve experiments, a set of standards containing a
range of known quantities. The standard dilution series is prepared by serially diluting
standards. For example, the standard stock is used to prepare the first dilution point, the first
dilution point is used to prepare the second dilution point, and so on. In the 7500/7500 Fast
system software, the volumes needed to prepare a standard dilution series are calculated by
the number of dilution points, the number of standard replicates, the starting quantity, the
serial factor, and the standard concentration in the stock. See also standard curve.
standard quantity A known quantity in the PCR reaction.
• In standard curve experiments, the quantity of target in the standard. In the
7500/7500 Fast system software, the units for standard quantity can be for mass, copy
number, viral load, or other units for measuring the quantity of target.
• In relative standard curve experiments, a known quantity in the standard. Standard
quantity can refer to the quantity of cDNA or the quantity of standard stock in the
PCR reaction. The units are not relevant for relative standard curve experiments
because they cancel out in the calculations.
starting quantity When defining a standard curve in the 7500/7500 Fast system software, corresponds to the
highest or lowest quantity.
step A component of the thermal profile. For each step in the thermal profile, you can set the
ramp rate (ramp increment for melt curve steps), hold temperature, hold time (duration),
and you can turn data collection on or off for the ramp or the hold parts of the step. For
cycling stages, a step is also defined by the AutoDelta status.
SYBR
®
Green
reagents
PCR reaction components that consist of two primers designed to amplify the target and
SYBR
®
Green dye to detect double-stranded DNA.

Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English