Applied Biosystems 7500 Fast Getting Started

Chapter 2 Design the Standard Curve Experiment
Set Up the Run Method
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Review the Run
Method Screen
1. Select either the Graphical View tab (default) or Tabular View tab.
2. Click the Reaction Volume Per Well field, then enter 25 µL.
3. Make sure the thermal profile displays the holding and cycling stages shown below.
If necessary, add a step, or click a temperature or time setting, then change it.
4. Click Next.
Design
Guidelines
When you design your own standard curve experiment:
• Enter a reaction volume/well. Applied Biosystems recommends a reaction volume
of 25 µL for standard curve experiments. The 7500 system supports reaction
volumes from 20 to 100 µL. The 7500 Fast system supports reaction volumes from
10 to 30 µL.
• Review the thermal profile:
– Make sure the thermal profile is appropriate for your reagents.
– If you are performing 1-step RT-PCR, include a reverse transcription step.
If your experiment requires a different thermal profile, edit the thermal profile or
replace the run method with one from the Run Method library. The Run Method
library is included in the 7500 software.
For More
Information
For more information on:
• The Run Method library or on completing the Run Method screen – Open the 7500
Software Help by clicking or pressing F1.
• Using Advanced Setup – See “Advanced Setup Workflow” on page 98.
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Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English