Chapter 2 Design the Standard Curve Experiment
Review the Reaction Setup
33
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
c. Review the components and calculated volumes for the PCR reactions:
7. In the Standard Dilution Series for RNase P TAMRA pane (see page 34):
a. Click the Standard Concentration in Stock field, then enter 20000.
b. Click the units field, then enter copies per µL.
Component Volume for 1 Reaction (µL)
Master Mix (2.0✕) 12.50
Assay Mix (20.0✕)1.25
Sample (10✕) or Standard 2.50
‡
‡ The sample or standard volume is limited to 10% of the total reaction volume.
H
2
O8.75
Total Volume 25.00
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2
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6b
6a
6c
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