Applied Biosystems 7500 - Page 15

Chapter 1 Introduction
About RQ Experiments
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 5
Notes
1
Required
User-Supplied
Materials
Example RQ Experiment
To better illustrate how to design, perform, and analyze RQ experiments, this section
guides you through an example experiment. The example experiment represents a
typical RQ experiment setup that you can use as a quick-start procedure to familiarize
yourself with the RQ workflow. Detailed steps in the RQ workflow are described in the
Item Source
ABI P
RISM
®
6100 Nucleic Acid PrepStation Applied Biosystems - (PN 6100-01)
High Capacity cDNA Archive Kit Applied Biosystems - (PN 4322171)
Taq Ma n
®
Universal PCR Master Mix
Taq Ma n
®
Fast Universal PCR Master Mix (2X)
No AmpErase
®
UNG
Applied Biosystems- (PN 4304437)
Applied Biosystems - (PN 4352042)
MicroAmp
®
Optical 96-Well Reaction Plate
Optical 96-Well Fast Thermal Cycling Plate
with Barcode (code 128)
Applied Biosystems - (PN 4306757)
Applied Biosystems - (PN 4346906)
Optical Adhesive Cover Applied Biosystems- (PN 4311971)
Labeled primers and probes from one of the
following sources:
•TaqMan
®
Gene Expression Assays
(predesigned primers and probes)
•TaqMan
®
Custom Gene Expression
Assays service (predesigned primers and
probes)
• Primer Express
®
Software (custom-
designed primers and probes)
• Applied Biosystems Web site
• Contact your Applied Biosystems Sales
Representative
Reagent tubes with caps, 10-mL Applied Biosystems
(PN 4305932)
Centrifuge with adapter for 96-well plates Major laboratory supplier (MLS)
Gloves MLS
Microcentrifuge MLS
Microcentrifuge tubes, sterile 1.5-mL MLS
Nuclease-free water MLS
Pipette tips, with filter plugs MLS
Pipettors, positive-displacement MLS
Safety goggles MLS
Vortexer MLS