Applied Biosystems 7500 Getting Started Guide

Chapter 6 Analyzing Data in an RQ Study
Creating an RQ Study Document
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 61
Notes
6
To create a new RQ Study document:
1. Select File> New.
2. In the Assay drop-down list of the New
Document Wizard, select Relative
Quantification (ddCt) Study. Accept the default
settings for Container and Template
(96-Well Clear and Blank Document).
3. Enter a name in the Default Plate Name field, or
accept the default.
4. Click Next>.
5. Add RQ plates to the study.
a. Click Add Plates.
Note: You can add up to 10 RQ plates to an
RQ study.
b. Select the plate(s) that you want to add to
the study, then click Open.
o
The selected plates are displayed.
IMPORTANT! All plates added to a study
must have identical thermal cycling
parameters—the same number of steps,
cycles, sample volume, emulation mode.
The SDS Software 1.3.1 will reject a plate if
it detects any differences. (The first plate
added to the study serves as the reference
plate against which other plates are
compared.)

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)