Applied Biosystems 7500 - Overview

Chapter 6 Analyzing Data in an RQ Study
Adjusting the Baseline and Threshold
66 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
Baseline Set Correctly
The amplification curve begins after the maximum
baseline. No adjustment necessary.
Baseline Set Too Low
The amplification curve begins too far to the right of
the maximum baseline. Increase the End Cycle value.
Baseline Set Too High
The amplification curve begins before the maximum
baseline. Decrease the End Cycle value.