Applied Biosystems 7500 Getting Started Guide

Chapter 6 Analyzing Data in an RQ Study
Adjusting the Baseline and Threshold
68 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
To manually adjust the baseline and threshold:
1. Select the Amplification Plot tab, then select Delta Rn vs. Cycle in the Data drop-
down list.
2. In the RQ Detector grid, select a detector.
The SDS Software 1.3.1 displays the:
• Associated samples (from all plates included in the study) in the RQ
Sample grid.
• Graph for the selected detector in the RQ Results panel.
Note: When manually adjusting baseline and threshold settings, you can select
only one detector at a time. If you select multiple detectors, the Analysis Settings
section and the threshold bar are disabled.
1
2
4b
exponential
phase of the
amplification
curve

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)