Applied Biosystems 7500 Getting Started Guide

Chapter 6 Analyzing Data in an RQ Study
Omitting Samples from a Study
76 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
5. Verify the uniformity of each replicate
population by comparing the groupings of C
T
values for the wells that make up the set.
Good
clustering
of replicate
data. No
outliers.
Potential
outlier.

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)