Applied Biosystems 7500 Getting Started Guide

Appendix B Example RQ Experiment
88 Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide
Notes
10. Analyze the RQ data, as explained in Chapter 6
on page 59.
a. Configure analysis settings ( ), using the
Auto Ct option and analyze the data.
Note: See “Configuring Analysis Settings”
on page 63 for details.
If you know the optimal baseline and
threshold settings for your experiment, you
can use the Manual Ct and Manual Baseline
options.
b. If necessary, manually adjust the baseline
and threshold.
Note: See “Adjusting the Baseline and
Threshold” on page 65.
c. Click , or select Analysis > Analyze to
reanalyze the data.
d. View analysis results by clicking a tab in the
RQ Results pane.
e. If desired, save the RQ Study document.
Conclusion
As shown in the figure on the right where liver is used
as the calibrator, expression levels of CCR2 are
greater in the liver than in the kidney or bladder
tissues of this individual.
Baseline is set before
amplification begins
Threshold falls within
exponential phase of curve

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)