Applied Biosystems 7500 Getting Started Guide

Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 89
References
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polymerase-catalyzed chain reaction. Methods Enzymol. 155:335–350.
Livak, K.J., and Schmittgen, T.D. 2001. Analysis of Relative Gene Expression Data
Using Real-Time Quantitative PCR and the 2
–∆∆C
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genomic sequences and restriction site analysis for diagnosis of sickle cell anemia.
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Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)