Chapter 4 --- Operation
48
How to Avoid Malfunctions
To cut usable sections, the following points are of utmost importance:
• Fit the knife edge, possibly move it horizontally to the left or right.
• Check adjustment of anti-roll plate guide and correct it, if necessary.
• Carefully remove debris from the front and rear part of the knife and from the anti-roll plate with the use of ethanol,
alcohol, or a similar reagent.
• Tighten all clamping screws and clamping levers on the knife carrier and specimen holder.
• Select the appropriate cutting temperature according to the specimen.
• Select the appropriate freezing medium.
N
ote
If
the specimen was frozen with liquid nitrogen or similar freezing techniques, the specimen must adapt itself to the cutting
temperature.
• Carefully select the requ
ired knife material and prole.
• Adjustment of knife height.
• Adjustment of
proper clearance angle. Usable cuts are only achieved at a clearance angle of 8-12°
or more.
• Take care in bringing the specimen and knife together.
Remove the specimens from the cooling chamber during defrosting, as the temperature inside the
c
hamber will increase. Do not leave or store tissue inside the over a longer period. Due to
p
ower failure or other unexpected malfunctions of the instrument, the specimen might be
d
amaged.
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