116 BD FACSCanto Flow Cytometer Reference Manual
efficiently direct the emitted light from each fluorochrome to a specific PMT,
through placement of LP mirrors and BP filters.
When the collected light leaves the fiber optic cable at the octagon, it first meets a
735 LP mirror (Figure A-9 on page 116). The mirror allows light with
wavelengths greater than 735 nm to pass and reflects light with lower
wavelengths to the next PMT.
Figure A-9 Light pathway around an octagon
Behind the LP mirror, a 780/60 BP filter admits light from 750 to 810 nm and
substantially blocks other wavelengths. The light that finally reaches PMT A will
be from dyes such as PE-Cy7 that emit in this range.
B
D
F
H
A
C
E
G
670LP
530/30
488/10
780/60
585/42
655LP
502LP
735LP
556LP
750–810 nm
> 670 nm
564–606 nm
515–545 nm
483–493 nm
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