Chapter 1: Introduction 17
The following table briefly describes these components.
When you install tubes onto the SIT, a pump within the fluidics cart pressurizes
the interior reservoir (plenum), which then provides sheath fluid to the flow cell.
At the same time, the sample tube is pressurized and sample is pushed up the SIT
and into the flow cell.
When you remove tubes from the SIT, the cytometer cleans the SIT by flushing
sheath solution down the inside and outside of the tube. The flushed sheath
solution is aspirated by the aspirator arm.
To activate SIT cleaning, push the aspirator arm all the way to the left when you
manually unload a tube. When you are using the Loader, SIT cleaning occurs
automatically.
Do not leave a tube of distilled water on the SIT between sample tubes, or during
or after daily shutdown.
flow cell where laser beam intercepts particles
tubing brings sheath and cleaning fluids to and aspirates waste from flow cell
SIT hollow metal tube that brings sample to flow cell
adapter lever lever used to change SIT from manual to automatic loading
aspirator arm movable waste aspiration port
aspirator arm
bar
metal bar used to push aspirator arm away from SIT during manual
loading
tube guide helps guide tube onto the SIT (Loader operation only)
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