18 BD FACSCanto Flow Cytometer Reference Manual
Flow Cell
Once the sample moves into the flow cell, particles move in single file past the
laser beams. The scattered and emitted light from these particles provides
information about their size, shape, granularity, and fluorescence properties.
The flow cell is beneath the flow cell access door. For more information, see
Fluidics System on page 106.
Figure 1-1 Flow cell
Optics
The optics system in the BD FACSCanto cytometer is composed of both
excitation optics and collection optics. Excitation optics bring light to the flow
cell. Collection optics gather the light signals emitted or scattered by the particles.
Excitation Optics
The excitation optics consist of lasers, fiber optic cables, beam-shaping prisms,
and an achromatic focusing lens, as shown in Figure 1-2 on page 20.
FSC diode
w
ere
asers intercept
sample stream
flow cell
obscuration bar
To Next Page
Loading...
