58 BD FACSCanto Flow Cytometer Reference Manual
3 Loosen the bleeder valve near the top of the filter by turning it
counterclockwise.
NOTICE Do not loosen the bottom bleeder valve. Ensure it is tightened.
; Tip Do not completely unscrew the valve. If you do, it will come off.
4 Wait until fluid seeps out.
Fluid should seep from the open valve within 30 seconds. If no fluid
appears, make sure the corresponding cubitainer is not empty or detached
from the cart. If the cubitainer contains fluid and the fluid lines are
attached and primed, the filter might be airlocked. See Removing an Air
Lock on page 72.
5 Close the valve by turning it clockwise.
6 Repeat steps 2 through 5 with the remaining filters.
loosen this
bleeder valve
DO NOT loosen
this bleeder valve
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